I have written a Matlab application,
frapfit, which performs basic analysis of a FRAP experiment. It does
simple exponential fitting providing the immobile fraction and the
empirical recovery time constant without calculating the diffusion
A tutorial about diffusion phenomena in cells and their investigation by FRAP is also available.
Registration: a machine specific code is shown when running the program for the first time, which you have to send to firstname.lastname@example.org. I will send you a machine-specific unlock code free of charge.
Installation: download frapfit.zip,
unzip it into a folder on your machine and type frapfit at the Matlab
command prompt after changing the current folder to the one you
installed the application into. Alternatively, add the folder to the
search path of Matlab.
DipImage, available free of charge, is required for running the program.
Help: the Help file also available from within the application.
Upgrading: The program will automatically check for and download upgrades.